CRYOPRESERVATION OF ATLANTIC CROAKER SPERMATOZOA Academic Article uri icon

abstract

  • An artificial insemination method was standardized. The effects of various extenders, dilution ratios, cryoprotectants, equilibration times, freezing and thawing rates, and semen and egg quantities on fertilization capacity of post-thaw Atlantic croaker Micropogonias undulatus spermatozoa were examined to optimize cryopreservation procedures. Fertilization rates using NaCl, Glucose, and sucrose solutions were comparable to those achieved with more complex extenders. No fertilization was obtained when methanol was the cryoprotectant. The optimum ratio of semen to DMSO to extender was 10%: 15%: 75%. No significant difference (P<0.05) in fertilization rates was found among freezing rates ranging from -10C/min to -150C/min. The two-step freezing method gave a fertilization rate similar to that obtained with the conventional one-step freezing method. 1991.

published proceedings

  • AQUACULTURE

author list (cited authors)

  • GWO, J. C., STRAWN, K., LONGNECKER, M. T., & ARNOLD, C. R.

citation count

  • 71

complete list of authors

  • GWO, JC||STRAWN, K||LONGNECKER, MT||ARNOLD, CR

publication date

  • May 1991