A membraneless continuous-flow filter for high-throughput separation and enrichment of particles and cells
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We describe a new microfluidic-based filtration method capable of performing simultaneous size-based separation and enrichment. Instead of forcing a particle or cell-laden suspension through a microporous membrane, microchannels are constructed with embedded weir-like barriers oriented parallel to the flow direction. When this geometry is incorporated into a curved flow path the resulting centrifugal forces act to push the suspended components across the barrier from the inner wall to the outer wall, with only those smaller than the barrier gap able to pass across. In addition to demonstrating extremely high selectivity, clogging effects are minimized because the primary flow acts to sweep aggregates downstream. We also describe a new lithography-free microfabrication approach to construct these barrier structures.
