Fish oil and pectin may suppress colon carcinogenesis via inhibition of the MAPK and TGF beta pathways Conference Paper uri icon


  • We have shown that fish oil/pectin(FP) diets are protective against colon cancer compared to corn oil/cellulose(CC). In this study we determined if the protection is conferred by influencing the temporal changes in expression of genes relevant to the MAPK and TGF pathway that are involved in colon carcinogenesis. Diets contained either FP or CC. Half of the rats were exposed to radiation (1 Gy, 1 GeV/nucleon iron) and all rats were injected with azoxymethane (2x, 15 mg/kg BW, sc) 10 and 17 d after irradiation. Exfoliated colonocyte polyA+ RNA was collected at 7, 14, and 28 wk in the same rats and gene expression determined using GE Codelink arrays. There were 301 differentially expressed genes for the diet X time interaction including five genes relevant to the MAPK pathway, e.g. expression of IL1was maintained over time in FPfed rats whereas expression in the 28 wk sample was 4.7 times (P=0.01) that in the 7 wk sample in CCfed rats. Also, three genes related to the TGF pathway were affected by the interaction, e.g. expression of Smad4, a tumor suppressor, was maintained over time in the FPfed rats but decreased at the tumor stage in CCfed rats (P=0.03). Our data indicate that a diet containing fish oil/pectin could suppress colon carcinogenesis by regulating expression of genes within the MAPK/TGF signaling axis. Funded by NSBRI NASA NCC 958, NIEHS (P30ES09106), and NIH (CA59034, CA57030).

published proceedings


author list (cited authors)

  • Cho, Y., Martinez, J. G., Turner, N. D., Taddeo, S. S., Davidson, L. A., Wang, N., ... Lupton, J. R.

citation count

  • 1

complete list of authors

  • Cho, Youngmi||Martinez, Josue G||Turner, Nancy D||Taddeo, Stella S||Davidson, Laurie A||Wang, Naisyin||Vannucci, Marina||Carroll, Raymond J||Chapkin, Robert S||Lupton, Joanne R

publication date

  • March 2008