Chemoprotective fish oil/pectin diets temporally alter gene expression profiles in exfoliated colonocytes Conference Paper uri icon


  • We have demonstrated that fish oil/pectin (FP) diets are protective against colon cancer compared to corn oil/cellulose (CC) in part by upregulating apoptosis at the promotion stage of carcinogenesis and suppressing peroxisome proliferatoractivated receptor (PPAR) expression at the tumor stage. To elucidate the underlying mechanisms whereby FP diets induce apoptosis and suppress PPAR signals, we analyzed the temporal gene expression profiles from exfoliated rat colonocytes. Rats consumed diets containing either FP or CC and were injected with azoxymethane (2x, 15 mg/kg BW, sc). Fecal material was collected at 7, 14, and 28 wk in the same rats and was used for poly A+ RNA extraction. Gene expression signatures were determined using GE Codelink arrays. GO analysis identified 10 genes upregulated by FP at the promotion stage of carcinogenesis involved in apoptosis (p=0.006). KEGG pathway analysis indicated that FP suppressed seven genes in the PPAR signaling pathway at the tumor stage (p=0.056). We conclude that the effects of chemotherapeutic diets on epithelial cell gene expression can be monitored noninvasively throughout the tumorigenic process.Grant Funding SourceFunded by NSBRI NASA NCC 958 and NIH (CA59034, CA57030).

published proceedings


author list (cited authors)

  • Cho, Y., Turner, N. D., Taddeo, S. S., Davidson, L. A., Wang, N., Vannucci, M., ... Lupton, J. R.

citation count

  • 0

complete list of authors

  • Cho, Youngmi||Turner, Nancy D||Taddeo, Stella S||Davidson, Laurie A||Wang, Naisyin||Vannucci, Marina||Carroll, Raymond J||Chapkin, Robert S||Lupton, Joanne R

publication date

  • April 2009