Proteome analysis of Escherichia coli K-12 by two-dimensional native-state chromatography and MALDI-MS Conference Paper

abstract

• The proteome analysis of Escherichia coli K-12 by two-dimensional native-state chromatography and MALDI-MS was described. It was observed that the cell respond to their environment with programmed changes in gene expression. Additional validation was accomplished by comparing hybridized RNA's generated from cells grown under identical conditions to proteins identified from this method. Utilizing changes in co-purification of proteins fractionated at varying pH suggests the components of higher order complexes.

authors

• Siegele, Deborah

published proceedings

• Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

altmetric score

• 1.5

author list (cited authors)

• Champion, M. M., Campbell, C. S., Siegele, D. A., Russell, D. H., & Hu, J. C

citation count

• 31

complete list of authors

• Champion, MM||Campbell, CS||Siegele, DA||Russell, DH||Hu, JC

publication date

• January 2002

Digital Object Identifier (DOI)

• 10.1046/j.1365-2958.2003.03294.x

start page

• 59

end page

• 60

URL

• http://dx.doi.org/10.1046/j.1365-2958.2003.03294.x