Location of fluorotryptophan sequestered in an amphiphilic nanoparticle by rotational-echo double-resonance NMR. Academic Article uri icon


  • Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been obtained of 6-fluorotryptophan complexed by a polymeric amphiphilic nanosphere consisting of a polystyrene core covalently attached to a poly(acrylic acid)-polyacrylamide shell. The REDOR spectra show that aromatic carbons from the polystyrene core and oxygenated carbons in the poly(acrylic acid)-polyacrylamide shell are both proximate to the 19F of 6-fluorotryptophan. Molecular modeling restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptophans are in the shell but within 10 A of the core-shell interface.

published proceedings

  • Biophys J

altmetric score

  • 3

author list (cited authors)

  • Baugher, A. H., Goetz, J. M., McDowell, L. M., Huang, H., Wooley, K. L., & Schaefer, J.

citation count

  • 24

complete list of authors

  • Baugher, AH||Goetz, JM||McDowell, LM||Huang, H||Wooley, KL||Schaefer, J

publication date

  • November 1998