Location of fluorotryptophan sequestered in an amphiphilic nanoparticle by rotational-echo double-resonance NMR. Academic Article

abstract

• Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been obtained of 6-fluorotryptophan complexed by a polymeric amphiphilic nanosphere consisting of a polystyrene core covalently attached to a poly(acrylic acid)-polyacrylamide shell. The REDOR spectra show that aromatic carbons from the polystyrene core and oxygenated carbons in the poly(acrylic acid)-polyacrylamide shell are both proximate to the 19F of 6-fluorotryptophan. Molecular modeling restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptophans are in the shell but within 10 A of the core-shell interface.

authors

• Wooley, Karen

published proceedings

• Biophys J

altmetric score

• 3

author list (cited authors)

• Baugher, A. H., Goetz, J. M., McDowell, L. M., Huang, H., Wooley, K. L., & Schaefer, J.

citation count

• 24

complete list of authors

• Baugher, AH||Goetz, JM||McDowell, LM||Huang, H||Wooley, KL||Schaefer, J

publication date

• November 1998

publisher

• Elsevier  Publisher

published in

• Biophysical Journal  Journal

keywords

• Acrylic Resins
• Carbon Isotopes
• Magnetic Resonance Spectroscopy
• Microscopy, Atomic Force
• Microspheres
• Molecular Conformation
• Molecular Structure
• Polystyrenes
• Tryptophan

Digital Object Identifier (DOI)

• 10.1016/S0006-3495(98)77702-1

start page

• 2574

end page

• 2576

volume

• 75

issue

• 5

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0006-3495%2898%2977702-1