MICROANALYSIS OF BIOMETALLIC COMPOUNDS USING NUCLEAR AND ATOMIC ACTIVATION Academic Article uri icon

abstract

  • A novel method for the characterization of metal containing biological compounds has been developed which utilizes both nuclear activation [e.g.,12C(3He, )11C] and atomic activations (X-ray emission) induced by 8 MeV3He bombardment. Detection limits for carbon (1 g), oxygen (1g), and metals (1 to 10 ng for elements between P and Zn) were obtained under routine experimental conditions. The metal stoichiometry of six different compounds (alkaline phosphatase, amylase, carbonic anhydrase, diamine oxidase, my oglobin, vitamin B12) were determined with typical relative precisions of 25% for a 40 g sample. A comparison of these ratios with carbon and metal measurements obtained by other methods showed a relative accuracy of 1 to 20%. 1979 Akadmiai Kiad.

published proceedings

  • JOURNAL OF RADIOANALYTICAL CHEMISTRY

author list (cited authors)

  • STOCK, G. J., & SCHWEIKERT, E. A.

citation count

  • 5

complete list of authors

  • STOCK, GJ||SCHWEIKERT, EA

publication date

  • March 1979