Defining Noncovalent Ubiquitin Homodimer Interfacial Interactions through Comparisons with Covalently Linked Diubiquitin. Academic Article

abstract

• Covalently linked diubiquitin (diUbq) is known to adopt specific interfacial interactions owing to steric hindrance induced by the covalent tether. K48-linked diUbq preferentially forms hydrophobic interfacial interactions between the two I44 faces under physiological conditions, whereas K63-linked diUbq preferentially forms electrostatic interfacial interactions. Here, we show using collision-induced unfolding ion mobility-mass spectrometry that the recently reported noncovalent dimer of ubiquitin exhibits structural preferences and interfacial interactions that are most similar to that of K48-linked diUbq.

authors

• Russell, David

published proceedings

• J Am Chem Soc

author list (cited authors)

• Wagner, N. D., & Russell, D. H.

citation count

• 11

complete list of authors

• Wagner, Nicole D||Russell, David H

publication date

• December 2016

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• 0306 Physical Chemistry (incl. Structural)

PubMed Central ID

• 27977175

Digital Object Identifier (DOI)

• 10.1021/jacs.6b09829

start page

• 16588

end page

• 16591

volume

• 138

issue

• 51