Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry. Academic Article

abstract

• MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.

authors

• Russell, David

published proceedings

• Peptides

author list (cited authors)

• Nachman, R. J., Russell, W. K., Coast, G. M., Russell, D. H., Miller, J. A., & Predel, R.

citation count

• 21

complete list of authors

• Nachman, Ronald J||Russell, William K||Coast, Geoffrey M||Russell, David H||Miller, J Allen||Predel, Reinhard

publication date

• March 2006

publisher

• Elsevier  Publisher

published in

• Peptides  Journal

keywords

• Amino Acid Sequence
• Animals
• Female
• Male
• Malpighian Tubules
• Muscidae
• Neuropeptides
• Neurosecretory Systems
• Oligopeptides
• Sequence Alignment
• Stimulation, Chemical

PubMed Central ID

• 16325965

Digital Object Identifier (DOI)

• 10.1016/j.peptides.2005.07.022

start page

• 521

end page

• 526

volume

• 27

issue

• 3

URL

• http://dx.doi.org/10.1016/j.peptides.2005.07.022