Optimization of a matrix-assisted laser desorption ionization-ion mobility-surface-induced dissociation-orthogonal-time-of-flight mass spectrometer: simultaneous acquisition of multiple correlated MS1 and MS2 spectra Academic Article uri icon

abstract

  • Optimization of a matrix-assisted laser desorption ionization-ion mobility-surface-induced dissociation-o-time-of-flight mass spectrometer for peptide sequencing is discussed. Surface-induced dissociation (SID) spectra obtained by using stainless steel, Au grids, and fluorinated self-assembled monolayers (F-SAM) on Au are compared. The F-SAM surfaces yield similar fragment ions to those obtained using an adventitious hydrocarbon coated stainless steel surface; however, optimum collision energies differ for the two surfaces. The advantage of ion mobility-time-of-flight (TOF) is the ability to simultaneously acquire MS1 and MS2 spectra, which greatly facilitates high-throughput sequencing of peptides and mixtures of peptides resulting form the proteolytic digest of proteins. Simultaneous acquisition of ion mobility and TOF spectra introduces a time element to SID experiments that can be used as a probe of ion/surface interactions. 2001 Elsevier Science B.V.

published proceedings

  • INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

author list (cited authors)

  • Stone, E. G., Gillig, K. J., Ruotolo, B. T., & Russell, D. H.

citation count

  • 34

complete list of authors

  • Stone, EG||Gillig, KJ||Ruotolo, BT||Russell, DH

publication date

  • November 2001