Peptide sequencing by MALDI 193-nm photodissociation TOF MS. Academic Article

abstract

• Ultraviolet photodissociation time-of-flight (TOF) mass spectrometry (MS) is described as a method for determination of peptide ion primary structure. Monoisotopic selection and bond-specific activation, combined with the rapidity of TOF MS analysis, render this technique invaluable to the rapidly expanding field of proteomics. Photofragment ion spectra of model peptides acquired using both post-source decay (PSD) focusing and TOF-TOF experimental methods are exhibited. Advantages of 193-nm photodissociation for de novo sequencing of peptide ions are discussed.

authors

• Russell, David

published proceedings

• Methods Enzymol

altmetric score

• 3

author list (cited authors)

• Morgan, J. W., Hettick, J. M., & Russell, D. H.

citation count

• 20

publication date

• January 2005

publisher

• Elsevier  Publisher

keywords

• Peptides
• Sequence Analysis, Protein
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Digital Object Identifier (DOI)

• 10.1016/S0076-6879(05)02006-9

start page

• 186

end page

• 209

volume

• 402

issue

• Anal. Chem.752003

URL

• http://dx.doi.org/10.1016/s0076-6879(05)02006-9