Coupling high-pressure MALDI with ion mobility/orthogonal time-of-flight mass spectrometry. Academic Article uri icon

abstract

  • A new ion mobility/time-of-flight mass spectrometer employing a high-pressure MALDI source has been designed and tested. The prototype instrument operates at a source/drift cell pressure of 1-10 Torr helium, resulting in a mobility resolution of approximately 25. A small time-of-flight mass spectrometer (20 cm) with a mass resolution of up to 200 has been attached to the drift cell to identify (in terms of mass-to-charge ratio) the separated ions. A simple tripeptide mixture has been separated in the drift tube and mass identified as singly protonated species. The ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared to results obtained on a high-vacuum time-of-flight instrument.

published proceedings

  • Anal Chem

altmetric score

  • 9

author list (cited authors)

  • Gillig, K. J., Ruotolo, B., Stone, E. G., Russell, D. H., Fuhrer, K., Gonin, M., & Schultz, A. J.

citation count

  • 146

complete list of authors

  • Gillig, KJ||Ruotolo, B||Stone, EG||Russell, DH||Fuhrer, K||Gonin, M||Schultz, AJ

publication date

  • September 2000