Size-selected (2-10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides. Academic Article

abstract

• In this report, first use of size-selected gold nanoparticles (AuNPs) as matrixes for matrix assisted laser desorption/ionization (MALDI) is described for peptides and proteins. In comparison with conventional organic acid MALDI matrixes, the optimum matrix-to-analyte ratio with AuNP matrixes is reduced by 10-14 orders of magnitude. Significant differences in the relative abundances of the ions observed in positive and negative mode MALDI-time-of-flight mass spectrometry (TOFMS) are revealed as the AuNP size distribution is decreased from 10 to 2 nm, whereby 2-nm AuNPs exhibit quantum confinement effects prevalent in quantum dots. AuNP matrixes allow for selective analyte ionization, as demonstrated in the selective MALDI-TOFMS of phosphotyrosine in a background of phosphoserine and phosphothreonine peptides.

authors

• Russell, David

published proceedings

• J Am Chem Soc

author list (cited authors)

• McLean, J. A., Stumpo, K. A., & Russell, D. H.

citation count

• 344

complete list of authors

• McLean, John A||Stumpo, Katherine A||Russell, David H

publication date

• April 2005

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Gold
• Nanostructures
• Oligopeptides
• Phosphopeptides
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Spectrophotometry, Ultraviolet

PubMed Central ID

• 15826152

Digital Object Identifier (DOI)

• 10.1021/ja043907w

start page

• 5304

end page

• 5305

volume

• 127

issue

• 15

URL

• http://dx.doi.org/10.1021/ja043907w