Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of paclitaxel and related taxanes. Academic Article uri icon

abstract

  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been demonstrated for the analysis of Taxus brevifolia extracts. The detection limit for the taxanes contained therein is estimated at 1 pmol using the matrix 4-nitroaniline at a matrix-to-analyte molar ratio of 100:1. Acquisition and calibration of the mass spectral data requires less than 5 min. The [M+H]+, [M+Na]+, and [M+K]+ ions provide mol wt confirmation and structurally significant fragment ions indicative of the various substituent groups located on the diterpene skeleton are formed.

published proceedings

  • J Nat Prod

altmetric score

  • 3

author list (cited authors)

  • Gimon, M. E., Kinsel, G. R., Edmondson, R. D., Russell, D. H., Prout, T. R., & Ewald, H. A.

citation count

  • 11

complete list of authors

  • Gimon, ME||Kinsel, GR||Edmondson, RD||Russell, DH||Prout, TR||Ewald, HA

publication date

  • October 1994