Identification of native flavin adducts from Fusarium oxysporum using accurate mass matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

abstract

• Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of a naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1-2 ppm) combined with an elemental composition search identifies the unknown FAD substituent as nitrobutane. The use of internal and external calibration in accurate mass MALDI-TOF is discussed.

authors

• Russell, David

published proceedings

• ANALYTICAL CHEMISTRY

author list (cited authors)

• Edmondson, R. D., Gadda, G., Fitzpatrick, P. F., & Russell, D. H.

citation count

• 14

complete list of authors

• Edmondson, RD||Gadda, G||Fitzpatrick, PF||Russell, DH

publication date

• July 1997

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

Digital Object Identifier (DOI)

• 10.1021/ac9700989

start page

• 2862

end page

• 2865

volume

• 69

issue

• 14

URL

• http://dx.doi.org/10.1021/ac9700989