Damping factor links periodic focusing and uniform field ion mobility for accurate determination of collision cross sections.
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abstract
The methodology for obtaining accurate ion-neutral collision cross section () values for peptides and proteins using periodic focusing ion mobility spectrometry (PF IMS) is presented. A mobility dampening factor (represented by the term ) is introduced to account for the relative increase in ion-neutral collisions in PF IMS compared to uniform field ion mobility spectrometry (UF IMS) for equivalent operating conditions. The results show that may be easily quantified both theoretically and empirically for a specific PF IMS design operating at a given pressure based upon the charge state of the analyte. By simply incorporating an term into traditional UF IMS expressions, accurate values were obtained with excellent agreement (4% difference) compared to UF IMS measurements found in the current literature.