UC/MALDI-MS analysis of HDL; evidence for density-dependent post-translational modifications.
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The purpose of this study is to determine whether the nature of the post-translational modifications of the major apolipoproteins of HDL is different for density-distinct subclasses. These subclasses were separated by ultracentrifugation using a novel density-forming solute to yield a high-resolution separation. The serum of two subjects, a control with a normolipidemic profile and a subject with diagnosed cardiovascular disease, was studied. Aliquots of three HDL subclasses were analyzed by MALDI and considerable differences were seen when comparing density-distinct subclasses and also when comparing the two subjects. A detailed analysis of the post-translational modification pattern of apoA-1 shows evidence of considerable protease activity, particularly in the more dense fractions. We conclude that part of the heterogeneity of the population of HDL particles is due to density-dependent protease activity.
author list (cited authors)
Johnson, J. D., Henriquez, R. R., Tichy, S. E., Russell, D. H., McNeal, C. J., & Macfarlane, R. D.
complete list of authors
Johnson, Jeffery D||Henriquez, Ronald R||Tichy, Shane E||Russell, David H||McNeal, Catherine J||Macfarlane, Ronald D