Photodissociation of high molecular weight peptides and proteins in a two-stage linear time-of-flight mass spectrometer. Academic Article uri icon


  • A two-stage linear time-of-flight mass spectrometer is used to investigate the requirements for performance of laser photodissociation of peptide and protein ions. Results are presented that demonstrate that desorption and dissociation laser pulses can be synchronized to irradiate ions that travel at high velocities down the drift tube of a time-of-flight mass spectrometer. For example, 193-nm photodissociation of bovine insulin and doubly charged lysozyme is demonstrated, and laser power studies suggest that dissociation is initiated by the absorption of a single 193-nm photon. These results are encouraging because they suggest that laser photodissociation of high molecular weight proteins can lead to fragmentation on time scales compatible with time-of-flight mass spectrometry.

published proceedings

  • J Am Soc Mass Spectrom

altmetric score

  • 6

author list (cited authors)

  • Gimon-Kinsel, M. E., Kinsel, G. R., Edmondson, R. D., & Russell, D. H.

citation count

  • 20

complete list of authors

  • Gimon-Kinsel, ME||Kinsel, GR||Edmondson, RD||Russell, DH

publication date

  • July 1995