A high repetition rate (1 kHz) microcrystal laser for high throughput atmospheric pressure MALDI-quadrupole-time-of-flight mass spectrometry. Academic Article

abstract

• Sample throughput has been increased in many areas of proteomics, but the last significant advance in lasers used for matrix-assisted laser desorption/ionization (MALDI) was the introduction of cartridge-type N2 lasers (337 nm, 4-ns pulse widths, 1-30-Hz repetition rates) more than a decade ago. This report describes the application of a 1-kHz repetition rate Nd:YAG laser (355 nm, <500-ps pulse widths) for atmospheric pressure MALDI-QqTOFMS, and data obtained are compared to a conventional nitrogen laser. For example, the signal intensity for angiotensin II using the 1-kHz laser was in some cases enhanced by a factor of 80 and high-quality data could be obtained in as little as 1 s.

authors

• Russell, David

published proceedings

• Anal Chem

author list (cited authors)

• McLean, J. A., Russell, W. K., & Russell, D. H.

citation count

• 36

complete list of authors

• McLean, John A||Russell, William K||Russell, David H

publication date

• February 2003

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

keywords

• 0299 Other Physical Sciences

PubMed Central ID

• 12585497

Digital Object Identifier (DOI)

• 10.1021/ac026029y

start page

• 648

end page

• 654

volume

• 75

issue

• 3

URL

• http://dx.doi.org/10.1021/ac026029y