FRAGMENTATION OF VITAMIN-B-12 DURING 337 NM MATRIX-ASSISTED LASER-DESORPTION IONIZATION Academic Article uri icon

abstract

  • Matrixassisted laser desorption ionization of vitamin B12 at 337 nm yields low abundances of the protonated molecule in timeofflight mass spectra when the matrix cyano4hydroxycinnamic acid is used. A variety of fragment ions are observed in the timeofflight mass spectra which may be assigned on the basis of previous studies of vitamin B12 fragmentation. When the matrix 4hydroxy3iodo5nitrobenzoic acid is used, an abundant signal from the protonated molecule ion of vitamin B12 is produced. Fragment ions are still observed in the timeofflight mass spectra but at much lower abundances. Studies of the influence of matrixtoanalyte ratio and desorbing laser intensity argue against direct photodissociation as the mechanism of fragment ion formation. Matrix choice appears to most strongly influence the internal energies of the laserdesorbed ions thereby controlling the fragmentation processes. Copyright 1994 John Wiley & Sons, Ltd.

published proceedings

  • BIOLOGICAL MASS SPECTROMETRY

altmetric score

  • 6

author list (cited authors)

  • KINSEL, G. R., PRESTON, L. M., & RUSSELL, D. H.

citation count

  • 35

complete list of authors

  • KINSEL, GR||PRESTON, LM||RUSSELL, DH

publication date

  • April 1994

publisher