Chemical Mechanism of the Phosphotriesterase from Sphingobium sp. Strain TCM1, an Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants. Academic Article uri icon


  • The mechanism of action of the manganese-dependent phosphotriesterase from Sphingobium sp. strain TCM1 that is capable of hydrolyzing organophosphate flame retardants was determined. The enzyme was shown to hydrolyze the RP-enantiomer of O-methyl O-cyclohexyl p-nitrophenyl thiophosphate with net inversion of configuration and without the formation of a covalent reaction intermediate. These results demonstrate that the enzyme catalyzes the hydrolysis of substrates by activation of a nucleophilic water molecule for direct attack at the phosphorus center.

published proceedings

  • J Am Chem Soc

author list (cited authors)

  • Bigley, A. N., Xiang, D. F., Ren, Z., Xue, H., Hull, K. G., Romo, D., & Raushel, F. M.

citation count

  • 22

complete list of authors

  • Bigley, Andrew N||Xiang, Dao Feng||Ren, Zhongjie||Xue, Haoran||Hull, Kenneth G||Romo, Daniel||Raushel, Frank M

publication date

  • February 2016