Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes. Academic Article

abstract

• A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

authors

• Liu, Wenshe

published proceedings

• Chem Commun (Camb)

author list (cited authors)

• Lee, Y., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R.

citation count

• 44

complete list of authors

• Lee, Yan-Jiun||Kurra, Yadagiri||Yang, Yanyan||Torres-Kolbus, Jessica||Deiters, Alexander||Liu, Wenshe R

publication date

• November 2014

publisher

• Royal Society of Chemistry (RSC)  Publisher

published in

• Chemical Communications  Journal

keywords

• Alkenes
• Amino Acids
• Amino Acyl-trna Synthetases
• Bacterial Outer Membrane Proteins
• Cycloaddition Reaction
• Escherichia Coli
• Escherichia Coli Proteins
• Fluorescein
• Fluorescent Dyes
• Green Fluorescent Proteins
• Heterocyclic Compounds, 1-ring
• Hydrolases
• Mutation
• RNA, Transfer

PubMed Central ID

• 25224663

Digital Object Identifier (DOI)

• 10.1039/c4cc06435f

start page

• 13085

end page

• 13088

volume

• 50

issue

• 86

URL

• http%3A%2F%2Fdx.doi.org%2F10.1039%2Fc4cc06435f