Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes. Academic Article uri icon


  • A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

published proceedings

  • Chem Commun (Camb)

author list (cited authors)

  • Lee, Y., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R.

citation count

  • 44

complete list of authors

  • Lee, Yan-Jiun||Kurra, Yadagiri||Yang, Yanyan||Torres-Kolbus, Jessica||Deiters, Alexander||Liu, Wenshe R

publication date

  • November 2014