Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes Academic Article uri icon


  • A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

author list (cited authors)

  • Lee, Y., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R.

citation count

  • 37

publication date

  • January 2014