A genetically encoded bidentate, metal-binding amino acid. Academic Article

abstract

• A two-ring binder: To facilitate the design of metalloproteins, the bidentate, metal-binding amino acid bipyridylalanine (BpyAla) was genetically encoded in E. coli in response to the amber nonsense codon with high fidelity and yield. The incorporation of BpyAla requires a BpyAla-specific aminoacyl-tRNA synthetase, which was evolved in a stepwise fashion. The structural basis of selective recognition of BpyAla by this synthetase was also determined. (Chemical Equation Presented). 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

authors

• Liu, Wenshe

published proceedings

• Angew Chem Int Ed Engl

altmetric score

• 3

author list (cited authors)

• Xie, J., Liu, W., & Schultz, P. G.

citation count

• 134

complete list of authors

• Xie, Jianming||Liu, Wenshe||Schultz, Peter G

publication date

• December 2007

publisher

• Wiley  Publisher

published in

• Angewandte Chemie International Edition  Journal

keywords

• Alanine
• Escherichia Coli
• Metalloproteins
• Metals
• Molecular Structure

Digital Object Identifier (DOI)

• 10.1002/anie.200703397

start page

• 9239

end page

• 9242

volume

• 46

issue

• 48

URL

• http%3A%2F%2Fdx.doi.org%2F10.1002%2Fanie.200703397