A genetically encoded bidentate, metal-binding amino acid. Academic Article uri icon


  • A two-ring binder: To facilitate the design of metalloproteins, the bidentate, metal-binding amino acid bipyridylalanine (BpyAla) was genetically encoded in E. coli in response to the amber nonsense codon with high fidelity and yield. The incorporation of BpyAla requires a BpyAla-specific aminoacyl-tRNA synthetase, which was evolved in a stepwise fashion. The structural basis of selective recognition of BpyAla by this synthetase was also determined. (Chemical Equation Presented). 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

published proceedings

  • Angew Chem Int Ed Engl

altmetric score

  • 3

author list (cited authors)

  • Xie, J., Liu, W., & Schultz, P. G.

citation count

  • 134

complete list of authors

  • Xie, Jianming||Liu, Wenshe||Schultz, Peter G

publication date

  • December 2007