A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum. Academic Article

abstract

• Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engineered pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs.

authors

• Liu, Wenshe

published proceedings

• J Am Chem Soc

altmetric score

• 10.5

author list (cited authors)

• Wang, Y., Fang, X., Wallace, A. L., Wu, B. o., & Liu, W. R.

citation count

• 107

complete list of authors

• Wang, Yane-Shih||Fang, Xinqiang||Wallace, Ashley L||Wu, Bo||Liu, Wenshe R

publication date

• February 2012

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Amino Acyl-trna Synthetases
• Binding Sites
• Electrophoresis, Polyacrylamide Gel
• Escherichia Coli
• Genetic Engineering
• Green Fluorescent Proteins
• Models, Chemical
• Mutation
• Nucleic Acid Conformation
• Peptides
• Phenylalanine
• Protein Engineering
• Pyrroles
• Substrate Specificity

PubMed Central ID

• 22289053

Digital Object Identifier (DOI)

• 10.1021/ja211972x

start page

• 2950

end page

• 2953

volume

• 134

issue

• 6

URL

• http%3A%2F%2Fdx.doi.org%2F10.1021%2Fja211972x