A facile method to synthesize histones with posttranslational modification mimics. Academic Article uri icon


  • Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

published proceedings

  • Biochemistry

altmetric score

  • 0.5

author list (cited authors)

  • Wang, Z. U., Wang, Y., Pai, P., Russell, W. K., Russell, D. H., & Liu, W. R.

citation count

  • 36

complete list of authors

  • Wang, Zhiyong U||Wang, Yane-Shih||Pai, Pei-Jing||Russell, William K||Russell, David H||Liu, Wenshe R

publication date

  • July 2012