A facile method to synthesize histones with posttranslational modification mimics. Academic Article

abstract

• Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

authors

• Liu, Wenshe
• Russell, David

published proceedings

• Biochemistry

altmetric score

• 0.5

author list (cited authors)

• Wang, Z. U., Wang, Y., Pai, P., Russell, W. K., Russell, D. H., & Liu, W. R.

citation count

• 36

complete list of authors

• Wang, Zhiyong U||Wang, Yane-Shih||Pai, Pei-Jing||Russell, William K||Russell, David H||Liu, Wenshe R

publication date

• July 2012

publisher

• American Chemical Society (ACS)  Publisher

published in

• Biochemistry  Journal

keywords

• Acetylation
• Histones
• Immunoprecipitation
• Lysine
• Methylation
• Molecular Structure
• Phosphorylation
• Protein Processing, Post-Translational
• Serine

PubMed Central ID

• 22697363

Digital Object Identifier (DOI)

• 10.1021/bi300535a

start page

• 5232

end page

• 5234

volume

• 51

issue

• 26

URL

• http://dx.doi.org/10.1021/bi300535a