Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids. Academic Article uri icon

abstract

  • The genetic incorporation of one azide-containing and one keto-containing noncanonical amino acids into a protein at one amber and one ochre mutation sites respectively followed by their orthogonal reactions with one hydroxylamine-containing and one cyclooctyne-containing dyes allows highly efficient one-pot site-specific dual labeling of the protein in a catalyst-free fashion.

published proceedings

  • Chembiochem

altmetric score

  • 3

author list (cited authors)

  • Wu, B. o., Wang, Z., Huang, Y., & Liu, W. R.

citation count

  • 63

complete list of authors

  • Wu, Bo||Wang, Zhiyong||Huang, Ying||Liu, Wenshe R

publication date

  • July 2012

publisher