Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid. Academic Article uri icon


  • The biosynthesis of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products typically involves a precursor peptide which contains a leader peptide that is important for the modification process, and that is removed in the final step by a protease. Genome mining efforts for new RiPPs are often hampered by the lack of a general method to remove the leader peptides. We describe here the incorporation of hydroxy acids into the precursor peptides in E. coli which results in connection of the leader peptide via an ester linkage that is readily cleaved by simple hydrolysis. We demonstrate the method for two lantibiotics, lacticin 481 and nukacin ISK-1.

published proceedings

  • J Am Chem Soc

altmetric score

  • 0.5

author list (cited authors)

  • Bindman, N. A., Bobeica, S. C., Liu, W. R., & van der Donk, W. A.

citation count

  • 32

complete list of authors

  • Bindman, Noah A||Bobeica, Silvia C||Liu, Wenshe R||van der Donk, Wilfred A

publication date

  • June 2015