E1-catalyzed ubiquitin C-terminal amidation for the facile synthesis of deubiquitinase substrates. Academic Article uri icon

abstract

  • Will Ub my partner? The ubiquitin (Ub)-activating enzyme (E1) was used to catalyze an amidation reaction to functionalize the C terminus of Ub with unique functional groups, such as thiol, azide, alkyne, and alkene groups, with high efficiency and yield (>90 %). These groups were then applied for the facile synthesis of fluorophore-conjugated ubiquitin and specifically conjugated diubiquitin substrates for deubiquitinases.

published proceedings

  • Chembiochem

altmetric score

  • 0.75

author list (cited authors)

  • Wang, X. A., Kurra, Y., Huang, Y., Lee, Y., & Liu, W. R.

citation count

  • 11

complete list of authors

  • Wang, Xiaoyan Aria||Kurra, Yadagiri||Huang, Ying||Lee, Yan-Jiun||Liu, Wenshe R

publication date

  • January 2014

publisher