From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis. Academic Article

abstract

• Bacteria and yeast utilize different strategies for sulfur incorporation in the biosynthesis of the thiamin thiazole. Bacteria use thiocarboxylated proteins. In contrast, Saccharomyces cerevisiae thiazole synthase (THI4p) uses an active site cysteine as the sulfide source and is inactivated after a single turnover. Here, we demonstrate that the Thi4 ortholog from Methanococcus jannaschii uses exogenous sulfide and is catalytic. Structural and biochemical studies on this enzyme elucidate the mechanistic details of the sulfide transfer reactions.

authors

• Begley, Tadhg
• Zhang, Xiuren

published proceedings

• J Am Chem Soc

altmetric score

• 0.5

author list (cited authors)

• Eser, B. E., Zhang, X., Chanani, P. K., Begley, T. P., & Ealick, S. E.

citation count

• 25

complete list of authors

• Eser, Bekir E||Zhang, Xuan||Chanani, Prem K||Begley, Tadhg P||Ealick, Steven E

publication date

• March 2016

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Catalysis
• Electron Spin Resonance Spectroscopy
• Ferric Compounds
• Ferrous Compounds
• Methanocaldococcus
• Models, Molecular
• Sulfides
• Thiamine
• Thiazoles

PubMed Central ID

• 26928142

Digital Object Identifier (DOI)

• 10.1021/jacs.6b00445

start page

• 3639

end page

• 3642

volume

• 138

issue

• 11

URL

• http%3A%2F%2Fdx.doi.org%2F10.1021%2Fjacs.6b00445