Cloning, sequencing and regulation of thiA, a thiamin biosynthesis gene from Bacillus subtilis. Academic Article

abstract

• The thiA gene is involved in the biosynthesis of the pyrimidine moiety of thiamin in Bacillus subtilis. This gene was cloned using a DNA probe rescued from thiA::Tn917 to screen a B. subtilis genomic library. ThiA exhibits 70% identity with E. coli thiC. The proposed thiA open reading frame complements all available thiA mutants of B. subtilis as well as the thiC mutant of E. coli. This suggests a similar biosynthetic pathway to 2-methyl-4-amino-5-hydroxymethylpyrimidine in both organisms. The expression of thiA is regulated, at least on the transcriptional level, and is significantly repressed by thiamin and 2-methyl-4-amino-5-hydroxymethylpyrimidine.

authors

• Begley, Tadhg

published proceedings

• Gene

altmetric score

• 3

author list (cited authors)

• Zhang, Y., & Begley, T. P.

citation count

• 16

complete list of authors

• Zhang, Y||Begley, TP

publication date

• January 1997

publisher

• Elsevier  Publisher

published in

• Gene  Journal

keywords

• Amino Acid Sequence
• Bacillus Subtilis
• Bacterial Proteins
• Base Sequence
• Cloning, Molecular
• DNA, Bacterial
• Escherichia Coli
• Genes, Bacterial
• Genetic Complementation Test
• Molecular Sequence Data
• Open Reading Frames
• Pyrimidines
• Sequence Alignment
• Sequence Homology, Amino Acid
• Thiamine

Digital Object Identifier (DOI)

• 10.1016/s0378-1119(97)00295-3

start page

• 73

end page

• 82

volume

• 198

issue

• 1-2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0378-1119%2897%2900295-3