PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099. Academic Article

abstract

• The function of the mlr6787 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 2-(acetamidomethylene)succinate hydrolase, an enzyme involved in the catabolism of pyridoxal 5'-phosphate (vitamin B 6). This enzyme was overexpressed in Escherichia coli, purified to homogeneity, and characterized. 2-(Acetamidomethylene)succinate hydrolase catalyzes the hydrolysis of 2-(acetamidomethylene)succinate to yield succinic semialdehyde, acetic acid, carbon dioxide, and ammonia. The k cat and K M for this reaction were 0.6 s (-1) and 143 microM, respectively. The enzyme was shown to utilize the E isomer of 2-(acetamidomethylene)succinate.

authors

• Begley, Tadhg

published proceedings

• Biochemistry

author list (cited authors)

• Mukherjee, T., Hilmey, D. G., & Begley, T. P.

citation count

• 11

complete list of authors

• Mukherjee, Tathagata||Hilmey, David G||Begley, Tadhg P

publication date

• June 2008

publisher

• American Chemical Society (ACS)  Publisher

published in

• Biochemistry  Journal

keywords

• Alphaproteobacteria
• Bacterial Proteins
• Cloning, Molecular
• DNA Primers
• Escherichia Coli
• Gamma-Aminobutyric Acid
• Hydrolases
• Kinetics
• Pseudomonas
• Pyridoxal Phosphate
• Recombinant Proteins
• Succinate-semialdehyde Dehydrogenase

PubMed Central ID

• 18476725

Digital Object Identifier (DOI)

• 10.1021/bi8004158

start page

• 6233

end page

• 6241

volume

• 47

issue

• 23

URL

• http%3A%2F%2Fdx.doi.org%2F10.1021%2Fbi8004158