Two-dimensional mass spectrometry of biomolecules at the subfemtomole level. Academic Article uri icon


  • Multiple dimensions of unique molecular structure information can now be obtained from proteins and DNA using mass spectrometry. Less than 10(-16) mol of the active major histocompatibility complex signaling peptide in a mixture of thousands can be identified. For large proteins (> 40 kDa), the high resolving power (> 10(5) and 10(-17) mol sensitivity of Fourier-transform mass spectrometry provide exact molecular weight values (+/- 1 or 2 Da) for mixture components, indicating error or modifications compared with the predicted DNA sequence. Selecting a specific molecular species, its two-dimensional spectrum indicates the part of the molecule that is modified; a three-dimensional spectrum of that fragment further isolates the modification site.

published proceedings

  • Curr Opin Chem Biol

author list (cited authors)

  • McLafferty, F. W., Kelleher, N. L., Begley, T. P., Fridriksson, E. K., Zubarev, R. A., & Horn, D. M.

citation count

  • 37

complete list of authors

  • McLafferty, FW||Kelleher, NL||Begley, TP||Fridriksson, EK||Zubarev, RA||Horn, DM

publication date

  • January 1, 1998 11:11 AM