Reagent for the detection of protein thiocarboxylates in the bacterial proteome: lissamine rhodamine B sulfonyl azide. Academic Article uri icon

abstract

  • Protein thiocarboxylates are involved in the biosynthesis of thiamin, molybdopterin, thioquinolobactin, and cysteine. Sequence analysis suggests that this post-translational modification is widely distributed in bacteria. Here we describe the development of lissamine rhodamine B sulfonyl azide as a sensitive click reagent for the detection of protein thiocarboxylates and describe the use of this reagent to detect PdtH, a putative protein thiocarboxylate involved in the biosynthesis of the pyridine dithiocarboxylic acid siderophore, in the Pseudomonas stutzeri proteome.

published proceedings

  • J Am Chem Soc

altmetric score

  • 1

author list (cited authors)

  • Krishnamoorthy, K., & Begley, T. P.

citation count

  • 17

complete list of authors

  • Krishnamoorthy, Kalyanaraman||Begley, Tadhg P

publication date

  • August 2010