Biosynthesis of a thiamin antivitamin in Clostridium botulinum. Academic Article uri icon


  • Bacimethrin-derived 2'-methoxythiamin pyrophosphate inhibits microbial growth by disrupting metabolic pathways dependent on thiamin-utilizing enzymes. This study describes the discovery of the bacimethrin biosynthetic gene cluster of Clostridium botulinum A ATCC 19397 and in vitro reconstitution of bacimethrin biosynthesis from cytidine 5'-monophosphate.

published proceedings

  • Biochemistry

author list (cited authors)

  • Cooper, L. E., O'Leary, S. E., & Begley, T. P.

citation count

  • 10

complete list of authors

  • Cooper, Lisa E||O'Leary, Se├ín E||Begley, Tadhg P

publication date

  • April 2014