A genetic screen for the identification of thiamin metabolic genes. Academic Article

abstract

• A genetic screen was developed for the identification of genes related to thiamin biosynthesis and degradation. Genes conferring resistance to bacimethrin or 4-amino-2-trifluoromethyl-5-hydroxymethylpyrimidine were selected from Escherichia coli and Bacillus subtilis genomic libraries. Hits from the selection included the known thiamin biosynthetic genes thiC, thiE, and dxs as well as five genes of previously unknown function (E. coli yjjX, yajO, ymfB, and cof and B. subtilis yveN). The gene products YmfB and Cof catalyze the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate. YmfB also converts thiamin pyrophosphate into thiamin phosphate.

authors

• Begley, Tadhg

published proceedings

• J Biol Chem

author list (cited authors)

• Lawhorn, B. G., Gerdes, S. Y., & Begley, T. P.

citation count

• 44

complete list of authors

• Lawhorn, Brian G||Gerdes, Svetlana Y||Begley, Tadhg P

publication date

• October 2004

publisher

• Elsevier  Publisher

published in

• Journal of Biological Chemistry  Journal

keywords

• Bacillus Subtilis
• Cloning, Molecular
• Escherichia Coli
• Genomic Library
• Phosphorylation
• Pyrimidines
• Thiamine

PubMed Central ID

• 15292217

Digital Object Identifier (DOI)

• 10.1074/jbc.M404284200

start page

• 43555

end page

• 43559

volume

• 279

issue

• 42

URL

• http%3A%2F%2Fdx.doi.org%2F10.1074%2Fjbc.m404284200