An assay for thiaminase I in complex biological samples. Academic Article

abstract

• An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.

authors

• Begley, Tadhg

published proceedings

• Anal Biochem

author list (cited authors)

• Hanes, J. W., Kraft, C. E., & Begley, T. P.

citation count

• 11

complete list of authors

• Hanes, Jeremiah W||Kraft, Clifford E||Begley, Tadhg P

publication date

• January 2007

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Alkyl And Aryl Transferases
• Complex Mixtures
• Hydrolases
• Kinetics
• Sensitivity And Specificity
• Spectrophotometry
• Substrate Specificity
• Sulfhydryl Compounds

Digital Object Identifier (DOI)

• 10.1016/j.ab.2007.06.001

start page

• 33

end page

• 38

volume

• 368

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.ab.2007.06.001