An assay for thiaminase I in complex biological samples.
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An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.
author list (cited authors)
Hanes, J. W., Kraft, C. E., & Begley, T. P.
complete list of authors
Hanes, Jeremiah W||Kraft, Clifford E||Begley, Tadhg P