An assay for thiaminase I in complex biological samples
- Additional Document Info
- View All
An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.
author list (cited authors)
Hanes, J. W., Kraft, C. E., & Begley, T. P.