An assay for thiaminase I in complex biological samples Academic Article uri icon

abstract

  • An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.

author list (cited authors)

  • Hanes, J. W., Kraft, C. E., & Begley, T. P.

citation count

  • 11

publication date

  • June 2007