Mechanistic studies on DNA photolyase, II. Is the blue enzyme isolated from Escherichia coli a mutant? Academic Article

abstract

• The contrasting properties of the DNA photolyases isolated from Escherichia coli, and from Saccharomyces cerevisiae suggested the possibility that the E. coli enzyme may have suffered mutagenesis as a consequence of the extensive use of ultraviolet irradiation/photoreactivation as a selection technique during the cloning. We have therefore recloned this gene using a UV-independent protocol and confirmed the original sequence.

authors

• Begley, Tadhg

published proceedings

• Mutat Res

author list (cited authors)

• Begley, T. P.

citation count

• 1

complete list of authors

• Begley, TP

publication date

• January 1991

publisher

• Elsevier  Publisher

published in

• MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS  Journal

keywords

• Cloning, Molecular
• Color
• Deoxyribodipyrimidine Photo-lyase
• Escherichia Coli
• Mutation
• Ultraviolet Rays

Digital Object Identifier (DOI)

• 10.1016/0165-7992(91)90127-p

start page

• 117

end page

• 118

volume

• 264

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2F0165-7992%2891%2990127-p