Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Academic Article

abstract

• A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

authors

• Begley, Tadhg

published proceedings

• Protein Sci

author list (cited authors)

• Kinsland, C., Taylor, S. V., Kelleher, N. L., McLafferty, F. W., & Begley, T. P.

citation count

• 43

complete list of authors

• Kinsland, C||Taylor, SV||Kelleher, NL||McLafferty, FW||Begley, TP

publication date

• August 1998

publisher

• Wiley  Publisher

published in

• Protein Science  Journal

keywords

• Electrophoresis, Polyacrylamide Gel
• Escherichia Coli
• Gene Expression
• Iodoacetic Acid
• Mass Spectrometry
• Models, Biological
• Models, Chemical
• Molecular Biology
• Recombinant Fusion Proteins
• Recombinant Proteins
• Sulfur Compounds
• Thiamine

PubMed Central ID

• 10082383

Digital Object Identifier (DOI)

• 10.1002/pro.5560070821

start page

• 1839

end page

• 1842

volume

• 7

issue

• 8

URL

• http%3A%2F%2Fdx.doi.org%2F10.1002%2Fpro.5560070821