Overexpression of recombinant proteins with a C‐terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis Academic Article uri icon

abstract

  • A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

author list (cited authors)

  • Kinsland, C., Taylor, S. V., Kelleher, N. L., Mclafferty, F. W., & Begley, T. P.

citation count

  • 38

publication date

  • August 1998

publisher