Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Academic Article uri icon


  • A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

published proceedings

  • Protein Sci

author list (cited authors)

  • Kinsland, C., Taylor, S. V., Kelleher, N. L., McLafferty, F. W., & Begley, T. P.

citation count

  • 43

complete list of authors

  • Kinsland, C||Taylor, SV||Kelleher, NL||McLafferty, FW||Begley, TP

publication date

  • August 1998