U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems. Academic Article uri icon


  • The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation to the dark blue 5,5'-dibromo-4,4'-dichloroindigo. Preliminary kinetic studies indicate that this compound may have practical use for assaying RNase A activity both in vitro and in vivo, e.g. in screening bacterial colonies for RNase A produced by recombinant DNA methods.

published proceedings

  • Nucleic Acids Res

altmetric score

  • 6

author list (cited authors)

  • Witmer, M. R., Falcomer, C. M., Weiner, M. P., Kay, M. S., Begley, T. P., Ganem, B., & Scheraga, H. A.

citation count

  • 22

complete list of authors

  • Witmer, MR||Falcomer, CM||Weiner, MP||Kay, MS||Begley, TP||Ganem, B||Scheraga, HA

publication date

  • January 1991