PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099.

abstract

• The function of the mlr6793 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 4-pyridoxic acid dehydrogenase, an enzyme involved in the catabolism of PLP (Vitamin B6). This enzyme was overexpressed in Escherichia coli and characterized. 4-Pyridoxic acid dehydrogenase is a 33kDa protein that catalyzes the four electron oxidation of 4-pyridoxic acid to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate, using nicotinamide adenine dinucleotide as a cofactor. The k cat for NADH production is 0.01s(-1). The KM values for 4-pyridoxic acid and NAD are 5.8 and 6.6microM, respectively.

authors

• Begley, Tadhg

published proceedings

• Bioorg Chem

author list (cited authors)

• Mukherjee, T., Kinsland, C., & Begley, T. P.

citation count

• 11

complete list of authors

• Mukherjee, Tathagata||Kinsland, Cynthia||Begley, Tadhg P

publication date

• January 2007

publisher

• Elsevier  Publisher

published in

• Bioorganic Chemistry  Journal

keywords

• Alcohol Oxidoreductases
• Chromatography, High Pressure Liquid
• Cloning, Molecular
• Escherichia Coli
• Pyridoxine
• Rhizobiaceae

PubMed Central ID

• 17868768

Digital Object Identifier (DOI)

• 10.1016/j.bioorg.2007.07.003

start page

• 458

end page

• 464

volume

• 35

issue

• 6

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.bioorg.2007.07.003