Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.
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TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.
author list (cited authors)
Jenkins, A. L., Zhang, Y., Ealick, S. E., & Begley, T. P.
complete list of authors
Jenkins, Amy L||Zhang, Yang||Ealick, Steven E||Begley, Tadhg P