Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis. Academic Article uri icon

abstract

  • TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.

published proceedings

  • Bioorg Chem

altmetric score

  • 3

author list (cited authors)

  • Jenkins, A. L., Zhang, Y., Ealick, S. E., & Begley, T. P.

citation count

  • 15

complete list of authors

  • Jenkins, Amy L||Zhang, Yang||Ealick, Steven E||Begley, Tadhg P

publication date

  • January 1, 2008 11:11 AM