Evidence for Binding of Dirhodium Bis-Acetate Units to Adjacent GG and AA Sites on Single-Stranded DNA Academic Article uri icon

abstract

  • Dirhodium tetraacetate is an antitumor active compound that is known to inhibit cellular DNA synthesis, but relatively little is known about its interactions with nucleic acids. A combination of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and enzymatic digestion experiments has established that the dirhodium bis-acetate unit forms an adduct with AA and GG sites in short DNA strands. The MALDI MS detection of the dirhodium/DNA adduct was aided by the addition of spermine, but no special sample treatment was required to obtain high quality mass spectra of the corresponding cisplatin-modified DNA strands.

author list (cited authors)

  • Asara, J. M., Hess, J. S., Lozada, E., Dunbar, K. R., & Allison, J

complete list of authors

  • Asara, John M||Hess, Jennifer S||Lozada, Elizabeth||Dunbar, Kim R||Allison, John

publication date

  • January 1, 2000 11:11 AM