The modular assembly of clusters is the natural synthetic strategy for the active site of [FeFe] hydrogenase. Academic Article uri icon


  • The 6Fe supercluster that comprises the [FeFe] hydrogenase active site has been shown to involve a modular buildup of ironsulfur clusters. Only three accessory proteins are needed to produce the CN, CO, and -SCH 2NHCH2S- units that bind to the 2Fe2S subcluster precursor. The scaffold/carrier protein inserts the preformed 2Fe subcluster into the apoprotein through a channel, which then closes to encapsulate the completed active site. 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

published proceedings

  • Angew Chem Int Ed Engl

author list (cited authors)

  • Bethel, R. D., Singleton, M. L., & Darensbourg, M. Y.

citation count

  • 17

complete list of authors

  • Bethel, Ryan D||Singleton, Michael L||Darensbourg, Marcetta Y

publication date

  • November 2010