Hyperfine interactions and electron distribution in Fe(II)Fe (I) and Fe (I)Fe (I) models for the active site of the [FeFe]hydrogenases: Mssbauer spectroscopy studies of low-spin Fe(I.). Academic Article uri icon


  • Mssbauer studies of [{-S(CH2C(CH3)2CH2S}(-CO)Fe(II)Fe(I)(PMe3)2(CO)3]PF6 (1 OX ), a model complex for the oxidized state of the [FeFe]hydrogenases, and the parent Fe(I)Fe(I) derivative are reported. The paramagnetic 1 OX is part of a series featuring a dimethylpropanedithiolate bridge, introducing steric hindrance with profound impact on the electronic structure of the diiron complex. Well-resolved spectra of 1 OX allow determination of the magnetic hyperfine couplings for the low-spin distal Fe(I) ([Formula: see text]) site, A x,y,z = [-24(6),-12(2), 20(2)]MHz, and the detection of significant internal fields (approximately 2.3T) at the low-spin ferrous site, confirmed by density functional theory (DFT) calculations. Mssbauer spectra of 1 OX show nonequivalent sites and no evidence of delocalization up to 200K. Insight from the experimental hyperfine tensors of the Fe(I) site is used in correlation with DFT to reveal the spatial distribution of metal orbitals. The Fe-Fe bond in [Fe2{-S(CH2C(CH3)2CH2S}(PMe3)2(CO)4] (1) involving two [Formula: see text]-type orbitals is crucial in keeping the structure intact in the presence of strain. On oxidation, the distal iron site is not restricted by the Fe-Fe bond, and thus the more stable isomer results from inversion of the square pyramid, rotating the [Formula: see text] orbital of [Formula: see text]. DFT calculations imply that the Mssbauer properties can be traced to this [Formula: see text] orbital. The structure of the magnetic hyperfine coupling tensor, A, of the low-spin Fe(I) in 1 OX is discussed in the context of the known Atensors for the oxidized states of the [FeFe]hydrogenases.

published proceedings

  • J Biol Inorg Chem

author list (cited authors)

  • Stoian, S. A., Hsieh, C., Singleton, M. L., Casuras, A. F., Darensbourg, M. Y., McNeely, K., Sweely, K., & Popescu, C. V.

citation count

  • 11

complete list of authors

  • Stoian, Sebastian A||Hsieh, Chung-Hung||Singleton, Michael L||Casuras, Andrea F||Darensbourg, Marcetta Y||McNeely, Kelsey||Sweely, Kurt||Popescu, Codrina V

publication date

  • August 2013