Production and purification of -amylase from hydrogen producing Enterobacter cloacae IIT-BT 08 Academic Article uri icon


  • Enterobacter cloacae IIT-BT08 was found to produce both -amylase and hydrogen in a batch system using soluble starch as substrate. Incubation time, temperature, pH and substrate concentration for the maximum -amylase activity (130 U/ml) were 8 h, 37 C, 6.00 and 10 g/l of soluble potato starch respectively. However, the optimum temperature and pH for the crude -amylase activity were 60 C and 4 respectively. The maximum rate of hydrogen production was observed at 10th h of fermentation and corresponding hydrogen yield was 7.6 mmol H2/g soluble potato starch.

published proceedings

  • Bioprocess and Biosystems Engineering

author list (cited authors)

  • Kumar, N., & Das, D.

citation count

  • 22

complete list of authors

  • Kumar, N||Das, D

publication date

  • January 2000