Photoacoustic imaging of single circulating melanoma cells in vivo
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2015 SPIE. Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.
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author list (cited authors)
Wang, L., Yao, J., Zhang, R., Xu, S., Li, G., Zou, J., & Wang, L. V.
complete list of authors
Wang, Lidai||Yao, Junjie||Zhang, Ruiying||Xu, Song||Li, Guo||Zou, Jun||Wang, Lihong V
editor list (cited editors)
Oraevsky, A. A., & Wang, L. V.