Genetic diversity among clonal isolates of Karenia brevis as measured with microsatellite markers Academic Article

abstract

• Karenia brevis is the major harmful bloom-forming dinoflagellate in the Gulf of Mexico yet little is known about the intraspecific genetic diversity of this species. Here we describe nine new microsatellite markers and, combined with nine previously described microsatellites, use them to genotype 40 cultured isolates of K. brevis. Genetic diversity identified from cultured isolates was compared with the genetic diversity identified from two field samples to assess how well the current cultures represent the field population. Thirty-nine unique haplotypes were identified from 40 cultured isolates of K. brevis using 18 microsatellite markers. Genetic diversity was similar between cultured isolates and the two field samples. The success of 18 microsatellite markers to distinguish individual isolates supports the use of microsatellites as a genetic tool for diagnostic identification of cultured isolates of K. brevis. 2012 Elsevier B.V.

authors

• Campbell, Lisa
• Henrichs, Darren

published proceedings

• HARMFUL ALGAE

author list (cited authors)

• Henrichs, D. W., Renshaw, M. A., Gold, J. R., & Campbell, L.

citation count

• 5

complete list of authors

• Henrichs, Darren W||Renshaw, Mark A||Gold, John R||Campbell, Lisa

publication date

• January 2013

publisher

• Elsevier  Publisher

keywords

• Dinoflagellate
• Gulf Of Mexico
• HAB
• Neurotoxic Shellfish Poisoning
• Population Genetics

Digital Object Identifier (DOI)

• 10.1016/j.hal.2012.11.003

start page

• 30

end page

• 35

volume

• 21-22

URL

• http://dx.doi.org/10.1016/j.hal.2012.11.003