3D Confocal Imaging of Pollen Academic Article uri icon


  • The study of pollen is important in paleoecology, paleontology, archeology, and forensics. The outer shell of a pollen grain bears characteristic features that help in determining the species or genus of origin. To view the pollen grain from several angles in a light microscope, the sample is usually mounted in glycerol and rolled on the microscopic slide by pushing the coverslip. For teaching, training, and also for establishing databases of pollen images, it is desirable to obtain three-dimensional datasets that allow rotation and viewing from any angle and reslicing in the computer.

author list (cited authors)

  • Vitha, S., Bryant, V. M., Zwa, A., & Holzenburg, A.

citation count

  • 1

publication date

  • March 2010