Anaerobic 5-Hydroxybenzimidazole Formation from Aminoimidazole Ribotide: An Unanticipated Intersection of Thiamin and Vitamin B Biosynthesis.
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Comparative genomics of the bacterial thiamin pyrimidine synthase (thiC) revealed a paralogue of thiC (bzaF) clustered with anaerobic vitamin B12 biosynthetic genes. Here we demonstrate that BzaF is a radical S-adenosylmethionine enzyme that catalyzes the remarkable conversion of aminoimidazole ribotide (AIR) to 5-hydroxybenzimidazole (5-HBI). We identify the origin of key product atoms and propose a reaction mechanism. These studies represent the first step in solving a long-standing problem in anaerobic vitamin B12 assembly and reveal an unanticipated intersection of thiamin and vitamin B12 biosynthesis.
author list (cited authors)
Mehta, A. P., Abdelwahed, S. H., Fenwick, M. K., Hazra, A. B., Taga, M. E., Zhang, Y., Ealick, S. E., & Begley, T. P.
complete list of authors
Mehta, Angad P||Abdelwahed, Sameh H||Fenwick, Michael K||Hazra, Amrita B||Taga, Michiko E||Zhang, Yang||Ealick, Steven E||Begley, Tadhg P