Genomic analysis of phase I and II Coxiella burnetii with restriction endonucleases.

abstract

• Restriction endonuclease-digested DNAs from several isolates of phase I and phase II Coxiella burnetii were compared using agarose gel electrophoresis and soft-laser scanning densitometry. Our results demonstrate that the two phases are, as previously assumed, alternative phases of the same organism. Although the restriction endonuclease digestion revealed genetic differences between clonal isolates of phase I and phase II C. burnetii Nine Mile strain, these differences do not appear to be related to antigenic phase variation. However, analyses of the fragment patterns generated by restriction enzyme digestion suggest potential grouping of the different isolates.

authors

• Samuel, James

published proceedings

• J Gen Microbiol

altmetric score

• 3

author list (cited authors)

• O'Rourke, A. T., Peacock, M., Samuel, J. E., Frazier, M. E., Natvig, D. O., Mallavia, L. P., & Baca, O.

citation count

• 15

complete list of authors

• O'Rourke, AT||Peacock, M||Samuel, JE||Frazier, ME||Natvig, DO||Mallavia, LP||Baca, O

publication date

• June 1985

publisher

• Microbiology Society  Publisher

published in

• Journal of general microbiology  Journal

keywords

• Coxiella
• DNA Restriction Enzymes
• DNA, Bacterial
• Densitometry
• Electrophoresis, Agar Gel
• Genes, Bacterial

PubMed Central ID

• 2995547

Digital Object Identifier (DOI)

• 10.1099/00221287-131-6-1543

start page

• 1543

end page

• 1546

volume

• 131

issue

• 6

URL

• http%3A%2F%2Fdx.doi.org%2F10.1099%2F00221287-131-6-1543